Microdetermination of Chloride in Blood Plasma and Cells, by Spectrophotometric Analysis Using Solid Silver Iodate.

نویسندگان

  • F J RODKEY
  • J SENDROY
چکیده

As an extension of the silver iodate method of Sendroy,a convenient, reproducible, and sensitive method for analysis of chloride in 0.02-mi. samples of serum, whole blood, or red cells isdescribed. The procedureinvolves (1) preparation of a proteinfree filtrate of the sample, (2) Ag103 reaction with the filtrate, and (3) spectrophotometric analysisof iodineas the quantitative measureof the amount of iodate exchangedfor chloride. Conditionsimportant for each of these stepshavebeen investigated. A precisionof ± 0.85 (S.D.) mM Cl per liter hasbeen found for this method, the results of which are within 1% of thoseobtained by the macro nitric acid digestion methodof Van Slyke.

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Microdetermination of Chloride in Biological Fluids, with Solid Silver Iodate

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عنوان ژورنال:
  • Clinical chemistry

دوره 12  شماره 

صفحات  -

تاریخ انتشار 1963