Microdetermination of Chloride in Blood Plasma and Cells, by Spectrophotometric Analysis Using Solid Silver Iodate.
نویسندگان
چکیده
As an extension of the silver iodate method of Sendroy,a convenient, reproducible, and sensitive method for analysis of chloride in 0.02-mi. samples of serum, whole blood, or red cells isdescribed. The procedureinvolves (1) preparation of a proteinfree filtrate of the sample, (2) Ag103 reaction with the filtrate, and (3) spectrophotometric analysisof iodineas the quantitative measureof the amount of iodate exchangedfor chloride. Conditionsimportant for each of these stepshavebeen investigated. A precisionof ± 0.85 (S.D.) mM Cl per liter hasbeen found for this method, the results of which are within 1% of thoseobtained by the macro nitric acid digestion methodof Van Slyke.
منابع مشابه
Microdetermination of Chloride in Biological Fluids, with Solid Silver Iodate
was outlined in detail, with gasometric measurement of the iodate resulting from the reaction. In this paper titrimetric measurement of the iodate is described. Titrimetric measurement of the iodate is directly applicable to the supernatant fluid in the AgI08 precipitation of chloride from salt solutions, urine, serum, and protein-free jiltrate of serum and of whole blood.’ For the titrimetric ...
متن کاملSpectrophotometric Simultaneous Kinetic Determination of Iodide and Iodate Using Partial Least-Squares Calibration Method in a Single Kinetic Run
A rapid, sensitive and versatile kinetic method is presented for the simultaneous spectrophotometric determination of iodide and iodate by partial least-squares regression (PLS) using original and derivate data named as absorbance and rate data. The method is based on the catalytic effect of the cited anions on the reaction rate between Ce(IV) and As(III) in 2 mol l?1 sulfuric acid medium. The ...
متن کاملPhotoelectric Microdetermination of Chloride in Biological Fluids, and of Iodate and Iodine in Protein-free Solutions
متن کامل
Partial Least Square and Parallel Factor Analysis Methods Applied for Spectrophotometric Determination of Cefixime in Pharmaceutical Formulations and Biological Fluid
In this study, the direct determination of cefixime as an anti-bacterial agent, in pharmaceutical formulations, urine and human blood plasma was conducted based on spectrophotometric measurements using parallel factor analysis (PARAFAC) and partial least squares (PLS). The calibration set was composed of fourteen solutions in the range of 0.50- 9.00 µg mL-1. PLS models were calculated at each p...
متن کاملPartial Least Square and Parallel Factor Analysis Methods Applied for Spectrophotometric Determination of Cefixime in Pharmaceutical Formulations and Biological Fluid
In this study, the direct determination of cefixime as an anti-bacterial agent, in pharmaceutical formulations, urine and human blood plasma was conducted based on spectrophotometric measurements using parallel factor analysis (PARAFAC) and partial least squares (PLS). The calibration set was composed of fourteen solutions in the range of 0.50- 9.00 µg mL-1. PLS models were calculated at each p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 12 شماره
صفحات -
تاریخ انتشار 1963